Dr. Gary Howard - Curriculum Vitae



Home Address:

107 Elm Court

Hammond, LA 70403

(985) 542-5984

Work Address:
Dept. of Biological Sciences
Southeastern Louisiana University
SLU10736
Hammond, LA 70402
Office: (985) 549-3501
Lab: (985) 549-2170
ghoward@selu.edu

Education:

B.S. Biology Western Kentucky University, 1985

M.S. Biology Western Kentucky University, 1987

Ph.D. Microbiology University of Illinois, 1992

Post-Doc Biochemistry Meharry Medical College, 1994

Experience:

1999- Present Assoc. Professor of Biology, Southeastern Louisiana University, Hammond, LA

1994 -1999 Assist. Professor of Biology, Southeastern Louisiana University, Hammond, LA

1993 -1994 Adjunct Part-Time Faculty, Cumberland University, Lebanon, TN

1992 -1994 Post-Doctoral Position, Dept. of Biochemistry, Meharry Medical College, Nashville, TN

1987 -1992 Research Assistant, Dept. of Animal Sciences, University of Illinois, Urbana, IL

1985 -1987 Teaching Assistant, Dept. of Biology, Western Kentucky University, Bowling Green, KY

Honors and Awards:

2002 International Biodeterioration & Biodegradation
Most downloaded articles April - December 2002
1. Gary T. Howard. Biodegradation of polyurethane: a review. International Biodeterioration & Biodegradation, 49 (2002) 245-252

2002 Who's Who Among America's Teachers

1999 Reviewer for International Biodeterioration & Biodegradation

1997 NSF (ROA) Visiting Research Professor at Clemson University

1987 1st Place Sigma Xi Graduate Research Award, Western Kentucky University

1985 1st Place Sigma Xi Undergraduate Research Award, Western Kentucky University

1985 Frank G. Brooks Award for Best Undergraduate Research in Southeastern Conference

Invited Talks:

Departmental Seminar, University of Illinois, Department of Animal Sciences, 1999
Departmental Seminar, Western Kentucky University, Department of Biology, 1999
NSF Sponsored Workshop on Biodeterioration of Construction Materials (Biological Deterioration of Plastics), University of Houston Department of Civil And Environmental Engineering, 1999
Key-Note Speaker at the Sigma Xi/ACS Annual Meeting in New Orleans, 1999
Departmental Seminar, Tulane University, Department of Biochemistry, 2000
Departmental Seminar, Louisiana State University, Department of Biology, 2001

Membership and Activities:

American Society for Microbiology
Associate Member, Sigma Xi
Gamma Sigma Delta
Tri Beta/Advisor
Council for Undergraduate Research

Grants Awarded:

Faculty Development Grants ($1,679) Genetic Characterization of Polyurethanase Genes from Pseudomonas fluorescens (1995)

Faculty Development Grants ($1,993) Effect of Surface Area to Volume Ratios on the Growth Rate Limiting First Order Saturation Kinetic Constants (1995)

LEQSF ($86,458) Molecular Characterization of Polyurethanase genes and Proteins from Pseudomonas fluorescens (1996-1997)

Faculty Develement Grant ($1,908) Biochemistry and Molecular Characterization of Sulfur Oxygenase from Thiobacillus neapolitanus (1996)

DOD ($508,804) Molecular Characterization of Polyurethanase Genes and Proteins from Pseudomonas (1996-1999)

LEQSF ($94,964) Characterization of the Enzymes Responsible for the Oxidation of Reduced Sulfur Compounds in Thiobacillus neapolitanus (1998-2000)

Faculty Development Grants ($2,000) Effect of Nitrogen Source on the Degradation of Louisiana Sweet Crude Oil by a Pseudomonas sp. (1998)

Faculty Development Grants ($2,000) Development and Evaluation of a Fluorescence Technique for Detection of Fecal Indicator Bacteria (1998)

LEQSF ($89,445) NMR for Enhancement of Undergraduate Education in Chemistry. (1998)

LEQSF ($96,712) Light Scattering Characterization of Enzymatic Degradation of Polyurethane (1999-2001)

Faculty Development Grants ($2,000) Evaluation of River Stage Height of the Tangipahoa River as an Early Warning Signal for Dangerous levels of Fecal Pollution (1999)

Faculty Development Grants ($2,000) Use of DGGE and PCR to Enhance Detection of Bacterial Fecal Pollution Using Species-Specific in situ Hybridization Probes (1999)

OSCAR Undergraduate Student Research Award ($1500) Molecular Characterization of
Polyurethanase genes from Anaerovibrio lipolytica

Faculty Development Grants ($2,000) Genetic analysis of the Polyurethanase Operon in Pseudomonas chlororaphis (2000)

Faculty Development Grants ($2,000) Analysis of Polyurethane Degrading Organisms in Soil Using Classical Methods and Denaturing Gradient Gel (2001)

Faculty Development Grants ($2,000) In situ Hybridization probes to detect Anaerobic bacteria (2001)

Faculty Technology Enhancement Award ($61,000) MIDI GC Sherlock System for Identification of Bacteria using FAME Analyses (2002)

LPBF-NOAA ($254,800) Assessment of Domestic and Agricultural Runoff in the Northern Boundaries of the Lake Pontchartrain Drainage Basin: A Survey of the Fecal Pollution Indicator Levels in the Tributaries and Lake Water and the Impact of Storm Water Runoff on the Naturally Occurring Microbial Communities Responsible for Nutrient Recycling (2002-2003)

Jefferson Parish Department of Environmental Affairs ($50,000) Assessment of microbial source tracking in Jefferson Parish coastal waters (2003)

USGS ($393,353) Development of a Bacterial Source Tracking System and Establishment of Total Maximum Daily Loads (TMDL) of Nutrients in the Northern Boundaries of the Lake Pontchartrain Drainage Basin (2003-2006)

Jefferson Parish Department of Environmental Affairs ($60,000) Assessment of microbial source tracking in Jefferson Parish coastal waters (2004)

LPBF-NOAA ($144,000) Baseline monitoring for environmental/ecological health indicators in the Joyce wildlife Management Area (JWMA): Preconstruction of the Wetland Assimilation Project, City of Hammond, LA. (2004- 2005)

Graduate Students/Post-Doctoral Students:

Rodney Vega

M.S. Dec. 1998
Outstanding Research Award. 1998 (Student Conference University of Houston at Clearlake)
Presentation at Annual Meeting of the American Society for Microbiology, Atlanta, GA, 1998
Research Assistant at Gensia-Sicor, Irvine , CA

Carmen Ruiz

B.S. May 1999
Undergraduate Research 1996-1999
Presentation at Student Conference University of Houston at Clearlake, 1998
Presentation at National Conference for Undergraduate Research, Rochester, NY, 1999
Presentation at Annual Meeting of the American Society for Microbiology, Miami, FL, 1997
Presentation at Annual Meeting of the American Society for Microbiology, Atlanta, GA, 1998
Presentation at Annual Meeting of the American Society for Microbiology, Chicago, IL, 1999
Research Assistant, Department of Biochemistry, LSU

Robert Stern

Ph.D. Biochemistry Florida State University
Post-Doc (1997-1999)

April Allen

M.S. Dec. 1999
Outstanding Research Award. 1998 (Student Conference University of Houston at Clearlake)
Presentation at Annual Meeting of the American Society for Microbiology, Atlanta, GA, 1998
Presentation at National Conference for Undergraduate Research, Rochester, NY, 1999
Research Assistant USDA, New Orleans

Tina Main Stricker

M.S. Dec. 1999
Presentation at Student Conference University of Houston at Clearlake, 1998
Presentation at Annual Meeting of the American Society for Microbiology, Atlanta, GA, 1998
Presentation at Annual Meeting of the American Society for Microbiology, Chicago, IL, 1999
Medical Science Officer Louisiana National Guard Unit

Jared Vicknair

M.S. Dec. 2000
Presentation at Student Conference University of Houston at Clearlake, 1998
Presentation at Annual Meeting of the American Society for Microbiology, Chicago, IL, 1999
Research Assistant, LSU Vet. Med. School

Phoebe Langlois

M.S. Dec. 2001
Presentation at Student Conference University of Houston at Clearlake, 2001
Ph.D. Student, UIUC, Urbana, IL

Lori Rowe

M.S. Dec. 2001
Presentation at Student Conference University of Houston at Clearlake, 2001
Research Assistant, Emory University, Atlanta, GA

Laura Peak

M.S. Dec. 2001
Presentation at Student Conference University of Houston at Clearlake, 2001
Research Assistant, Department of Microbiology LSU, Baton Rouge, LA

Virgil Tiller

M.S. Dec. 2003
Ph.D. Student, Tulane University, New Orleans, LA

Melanie Mask

M.S. Dec. 2002
Research Assistant USDA, Oxford, MS

Bronwyn Duos

M.S. Dec 2002
Research Assistant, Southeastern Louisiana University, Hammond, LA
Research Assistant, Pennington Institute, Baton Rouge, LA

Karam Aboudeh

M.S. May 2005
Ph.D. Student, Tulane University, New Orleans, LA


Publications:

Howard, G.T. 1986. Germination of the seeds of Euonymus americanus L. with the use of rumen fluid of cows. Bios 57: 14-18.

Howard, G.T. and L.P. Elliott. 1988. Effects of cellulolytic ruminal bacteria and of cell extracts on germination of Euonymus americanus L. seeds Appl. Environ. Microbiol. 54: 218-224.

Howard, G.T. and B. A White. 1988. Molecular cloning and expression of cellulase genes from Ruminococcus albus 8 in Escherichia coli bacteriophage ?. Appl. Environ. Microbiol. 54: 1752-1755.

Howard, G.T. and B. A White. 1990. Cloning in Escherichia coli of a bifunctional cellulase/xylanase enzyme from Ruminococcus flavefaciens FD-1. Animal Biotechnology. 1: 95-106.

White, B.A., J.H. Clarke, K.C. Doerner, V.K. Gupta, C.T. Helaszek, G.T. Howard, M. Morrison, A.A. Odenyo, S. Rosenzweig, and R.I. Mackie. 1990. Improving cellulase activity in Ruminococcus through genetic modification, p. 389-400. In D.E. Akin, L.G. Ljungdahl, J.R. Wilson, and P.J. Harris (ed), Microbial and Plant Opportunities to Improve Lignocellulose Utilization by Ruminants. Elsevier Science Publishing Co., New York.

Doerner, K.C., G.T. Howard, R.I. Mackie, and B.A. White 1992. ß-Glucanase expression by Ruminococcus flavefaciens FD-1. FEMS Microbiol. Lett. 93: 147-154.

Blake, R.C., G.T. Howard, and S. McGinness. 1994. Enhanced yields of iron-oxidizing bacteria by in situ electrochemical reduction of soluble iron in the growth medium. Appl. Environ. Microbiol. 60: 2704-2710.

Blake, R.C., E.A. Shute, and G.T. Howard. 1994. Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur. Appl. Environ. Microbiol. 60: 3349-3357.

W. Norton and G. Howard. 1995. Ultrastructural analysis of the physical interactions that occur between Bacillus sp and polyurethane during biodegradation. Proceeding of the Microscopy Society of America. 53: 345-346.

Blake, R.C. and G.T. Howard. 1998. Adhesion and growth of a Bacillus sp. on a polyesterurethane. International Biodeterioration and Biodegradation. 42: 63-73.

Baker, S.H., S. Jin, H.C. Aldrich, G.T. Howard, and J.M. Shively. 1998. Insertion Mutation of the form I cbbL of RuBisCO in Thiobacillus neapolitanus results in the expression of form II RubisCO, the loss of carboxysomes, and an increased CO2 requirement for growth. J. Bacteriol. 180: 4133-4139.

Howard, G.T. and R.C. Blake. 1999. Growth of Pseudomonas fluorescens on a polyester-polyurethane and the purification and characterization of a polyurethanase-protease enzyme. International Biodeterioration and Biodegradation. 42: 213-220.

Ruiz, C., N. Hilliard, and G.T. Howard. 1999. Growth of Pseudomonas chlororaphis on a polyester-polyurethane and the purification and characterization of a polyurethanse-esterase enzyme. International Biodeterioration and Biodegradation. 43: 7-12.

Howard, G.T. and N.P. Hilliard. 1999. Use of coomassie blue-polyurethane interaction in detection of polyurethanease proteins and polyurethanolytic bacteria. Int. Bioterioration Biodegradation 43:23-30

Allen, A., N. Hilliard, and G.T. Howard. 1999. Purification and characterization of a soluble polyurethane degrading enzyme from Comamonos acidovorans. International Biodeterioration and Biodegradation. 43: 37-41.

Ruiz, C.,T. Main, N. Hilliard, and G.T. Howard. 1999. Purification and characterization of two polyurethanse enzymes from Pseudomonas chlororaphis.. International Biodeterioration and Biodegradation. 43: 43-47.

Vega, R., T. Main, and G.T. Howard. 1999. Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens. International Biodeterioration and Biodegradation. 43: 49-55.

Ruiz, C. and G.T. Howard. 1999. Nucleotide sequencing of a polyurethanase gene (pulA) from Pseudomonas fluorescens. International Biodeterioration and Biodegradation. 44: 127-131.

Stern, R.S. and G.T. Howard. 2000. The polyester polyurethane gene (pueA) from Pseudomonas chlororaphis encodes a lipase. FEMS Microbiological Letters. FEMS Microbiol Lett. 185: 163-168.

Howard, G.T., B. Crother, and J. Vicknair. 2001. Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis. International Biodeterioration and Biodegradation. 47:17-25.

G.T. Howard, J. Vicknair and R.I. Mackie. 2001. Sensitive plate assay for screening and detection of bacterial polyurethanase activity. Letters in Applied Microbiology. 32:211-214.

Lori Rowe and G.T. Howard. 2002. Growth of Bacillus subtilis on polyurethane and the purification and characterization of a polyurethanase-lipase enzyme. International Biodeterioration and Biodegradation. 50: 33-40.

Phoebe Langlois and G.T. Howard. 2002. A single glycine-rich repeat of Pseudomonas chlororaphis Polyurethanase A mediates secretion of a GST fusion protein in Escherichia coli. International Biodeterioration and Biodegradation. 50:135-140

Howard, G.T. 2002. Biodegradation of Polyurethane: A Review. International Biodeterioration and Biodegradation. 49:245-252

Abstracts:

Howard, G.T. 1985. Germination of the seeds of Euonymus americanus L. from south central Kentucky with the use of bovine rumen fluid. Abstr. 28th Assoc. Southeastern Biologists.

Hill, D.R., J.R. McCurry, L.P. Elliott, and G.T. Howard. 1985. SEM observations on the germination of Euonymus americanus. Pro 43rd Ann. Meet Electctron Microscopy Soc. Amer.

Howard, G.T. and L.P. Elliott. 1986. Germination of seeds of Euonymus americanus with the use of Clostridium cellobioparum. Trans KY Acad. Sci.

Howard, G.T. and L.P. Elliott. 1986. Germination of seeds of Euonymus americanus with the cellulase from Clostridium cellobioparum. Abstr. Ann. Amer Soc. Microbiol.

Howard, G.T. and B.A. White. 1987. Molecular cloning and expression of cellulase genes from Ruminococcus albus 8 in Escherichia coli bacteriophage ?. Pro 19th Biennial Conference on Rumen Function.

White, B.A and G.T. Howard. 1987. Molecular cloning and expression of endoglucanase genes from Ruminococcus flavefaciens FD-1 in Escherichia coli bacteriophage ?. Pro 19th Biennial Conference on Rumen Function.

Howard, G.T. and B.A. White. 1988. Cloning of cellulase genes from Ruminococcus albus and Ruminococcus flavefaciens. Abstr. Ann. Meet. Amer. Soc. Microbiol.

White, B.A., G.T. Howard, S. Rosenzweig, and J.H. Clarke. 1989. Cloning of an endoglucanase gene from Ruminococcus flavefaciens FD-1. Proc. 20th Biennial Conference on Rumen Function.

Gupta, V.K., G.T. Howard, S. Rosenzweig, and B.A. White. 1989. More cloning of endoglucanase genes from Ruminococcus flavefaciens FD-1. Proc. 20th Biennial Conference on Rumen Function.

Blake, R.C. and G.T. Howard. 1994. Binding of Thiobacillus ferrooxidans with its insoluble substrate. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Simpson, J.M. G.T. Howard, K. Bancroft, and G.W. Childers.1995. Bioremediation for small scale oil spills in Louisiana marshes. Abstr. Ann. Meet. Amer. Soc. Microbiol. South Central Branch

Howard, G.T. and R.C.Blake. 1996. Characterization of growth and a polyurethanase protein from Pseudomonas fluorescens on a polyesterurethane. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Simpson, J.M. G.T. Howard, K. Bancroft, and G.W. Childers.1996. Bioremediation for small scale oil spills in Louisiana marshes. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Howard, G.T., S.C. Lorbach, R.C. Blake. 1996. Isolation of a polyurethane-degrading enzyme from Pseudomonas putida. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Ferachi, R.P., N. Hilliard, and G.T. Howard. 1996. Analysis of urethane from microbial extracts. Abstr. Louisiana Academy of Sciences

Ruiz, C. and G. T. Howard. 1997 Characterization of Growth and a Polyurethanase protein from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Thurmond, J., W.N. Norton, and G.T. Howard.1997. Growth and Adhesion on Airplane Waste by a Pseudomonas. Abstr. Ann. Meet. Amer. Soc. Microbiol. South Central Branch

Howard,G.T. and N.P. Hilliard. 1998. Use of Coomassie Blue-Polyurethane Interactions in Detection of Polyurethanase proteins and Polyurethanolytic Bacteria. Louisiana Academy of Sciences.

Hilliard, N.P. and G.T. Howard. 1998. Chromophore production during the degradation of polyurethanes by Pseudomonas sp. Louisiana Academy of Sciences

Main, T., N. Hilliard, G. Howard. 1998. Purification and Characterization of a polyurethanse from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Allen, A.B., N.P. Hilliard, and G.T. Howard. 1998. Purification and characterization of a polyurethane degrading enzyme from Comamonas acidovorans. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Stern, R.V. and G.T. Howard. 1998. Cloning and expression of a polyurethane gene from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Ruiz, C.R., N.P. Hilliard, and G.T, Howard. 1998. Purification and characterization of a polyurethanse from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Vega, R.E. and G.T. Howard. 1998. Cloning and expression of a polyurethanse gene from Pseudomonas fluorescens. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Thurmond, J., W.N. Norton, and G.T. Howard. 1998. Utilization of airplane paint waste as a nutrient source by Pseudomonas sp. VM1. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Vega, R.E. and G.T. Howard. 1998. Cloning and expression of a polyurethanse gene from Pseudomonas fluorescens. Ann. Meet. Amer. Soc. Microbiol. South Central Branch

Ruiz, C. and G.T. Howard. 1999. Nucleotide sequencing and transcriptional analysis of a polyurethanase gene from Pseudomonas fluorescens . Abstr. Ann. Meet. Amer. Soc. Microbiol.

Stern, R. and G.T. Howard. 1999. Nucleotide sequence analysis and overexpression of the polyurethanase encoded by the gene (pueA) from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Main, T. and G.T. Howard. 1999. Purification and characterization of a polyurethanase from Pseudomonas fluoirescens. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Vicknair, J.S. and G.T. Howard. 1999. Cloning and expression of a polyurethanase gene from Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Jones, B.C., N. Hilliard, and G.T. Howard. 1999. Isolation and characterization of a thiosulfate oxiding activity from Thiobacillus neapolitanus. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Himel,D., D. Norwood, and G. Howard. 2000. Light scattering study of enzymatic degradation of polyurethane. Abstr. Ann. Meet. Amer. Physics Soc. Southeastern Section.

Duos, B and G. T. Howard 2003. The Effects of multiple copies of the pueA gene, and the molecular characterization of the pueB gene in Pseudomonas chlororaphis. Abstr. Ann. Meet. Amer. Soc. Microbiol.

S. Ohene-Adjei, R. Mackie, G. Howard, G. Childers, B. Duos, Mary Miller, and I. Cann.2004. Diversity of Archaeal Communities in Lake Ponchatrain Basin: Impact of Salt and Sulfate Gradients. Abstr. Ann. Meet. Amer. Soc. Microbiol.

Duos, B and G. T. Howard 2004. Kinetic Exclusion Assays to Quantify PueA Binding Interactions in PolyurethaneAbstr. Ann. Meet. Amer. Soc. Microbiol.

C.J. Schulz, G.W. Childers, and G. T. Howard. 2004. PCR based detection and survival studies of potential microbial source tracking markers for species-specific fecal pollution indicators. LPB

G.W. Childers, C.J. Schulz, and G. T. Howard.2004.. Preliminary studies of E. coli sedimentation and re-growth in Tangipahoa River, Louisiana, Waters. LPB.